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A complex study of biological effects of some soya products
Ručková, Michaela ; Němcová, Andrea (referee) ; Márová, Ivana (advisor)
Soybeans and products thereof are considered as a very contradictory legume across layman and expert opinions. Some see it almost as a “superfood” while others avoid it due to its biologically active content with an unclear effect on the organism. As a lot of research on soybeans was already performed the content varies considerably and it is not easy to grasp the issue correctly. The objective of the research part of the thesis is a structuralized study of already published scientific knowledge to clarify the current state of the art. Experimental part of the thesis focuses on characterisation of soy and its products in terms of active ingredients content and determination of antioxidants and antimicrobial effect. After that, the cytotoxic effect on human heterogeneous colorectal adenocarcinoma epithelial cell line and mouse melanoma cell line is determined by in vitro testing. All obtained information and results of experimental part could possibly serve as a starting point for further study of soy and soy products, both in the dissertation and in the submission of a grant project.
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A complex study of biological effects of some soya products
Ručková, Michaela ; Němcová, Andrea (referee) ; Márová, Ivana (advisor)
Soybeans and products thereof are considered as a very contradictory legume across layman and expert opinions. Some see it almost as a “superfood” while others avoid it due to its biologically active content with an unclear effect on the organism. As a lot of research on soybeans was already performed the content varies considerably and it is not easy to grasp the issue correctly. The objective of the research part of the thesis is a structuralized study of already published scientific knowledge to clarify the current state of the art. Experimental part of the thesis focuses on characterisation of soy and its products in terms of active ingredients content and determination of antioxidants and antimicrobial effect. After that, the cytotoxic effect on human heterogeneous colorectal adenocarcinoma epithelial cell line and mouse melanoma cell line is determined by in vitro testing. All obtained information and results of experimental part could possibly serve as a starting point for further study of soy and soy products, both in the dissertation and in the submission of a grant project.
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Cytochemická vyšetření akvitivity nukleolů po podání genisteinu na modelovém objektu
PICMONOVÁ, Veronika
This thesis deals with exploration of the effect of genistein on proteosynthetic activity of nucleoli by observing changes in their morphology. The effect of genistein on the function of nucleoli was observed in hemocytes of the experimental animal Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae). 4 types of hemocytes were found in the hemolymph of S. littoralis (Boisduval): granulocytes, prohemocytes, plasmatocytes and spherulocytes. The effect of genistein on individual types and concentration of hemocytes is not clear. Genistein was applied in doses of 0,135 mg and 0,54 mg per larva, and subsequently changes in morphology, number and size of nucleoli were examined upon 8 and 24 hours. Furthermore, changes in morphology of the hemocytes were examined, as well as their concentration using toluidine blue staining, silver staining, panoptic staining and using the Türk{\crq}s solution. For the purpose of changes assessment, the material was processed using an optical microscope and the computer software Nis Element AR 2.30. In the course of observation, changes in the number of nucleoli were found upon application of genistein. The number of compact nucleoli and micronucleoli increased. On the contrary, the number of ring-shaped nucleoli decreased. The area of nucleoli increased upon application of lower dosage of genistein (0,135 mg); however, upon action of the higher dose (0,54 mg), it decreased, on the contrary. Nucleolar index values remained without rather significant changes. In the biomodel used, genistein evoked changes which can be viewed as immune responses from the viewpoint of hematotoxicology.
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